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KMID : 1100720130330020121
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Annals of Laboratory Medicine
2013 Volume.33 No. 2 p.121 ~ p.124
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Comparison of 3 Automated Immunoassays for Detection of Anti-Hepatitis A Virus Immunoglobulin M in a Tertiary Care Hospital
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Park Hye-Won
Lee Yu-Joo
Seong Moon-Woo
Park Myoung-Hee
Song Eun-Young
Lee Do-Hoon
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Abstract
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Three automated immunoassay kits for anti-Hepatitis A Virus (HAV) IgMratories, USA), Elecsys (Roche Diagnostics, Germany), and ADVIA Centaur (Siemens Healthcare Diagnostics Inc., USA)78 consecutive samples, for which an anti-HAV IgM test was requested at Seoul National University Hospital from September 2009 to January 2010. Reviewing of medical records, reverse transcription (RT)-PCR for HAV RNA, or total anti-HAV assay were performed on 16 (9.0%) samples with discrepant results. The percent agreements (kappas) of the Architect and ADVIA Centaur, Architect and Elecsys, and ADVIA Centaur and Elecsys kits were 96.6% (0.91), 96.6% (0.92), and 97.8% (0.94), respectively. Eight out of 16 discrepant samples showed gray-zone values in Architect but were nonreactive in the others. Slightly earlier seroconversion was suspected in Elecsys. The 3 assays showed comparable performances with excellent agreements in a tertiary care hospital setting.
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KEYWORD
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Anti-hepatitis A virus immunoglobulin M, Immunoassay, Agreement
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